[01] Lyudmila Zaynitdinova, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan. [02] Noira Vokhidova, Institute of Chemistry and Physics of Polymers, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan. [03] Javlon Tashpulatov, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan. [04] Svetlana Kukanova, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan. [05] Nurbek Ashurov, Institute of Chemistry and Physics of Polymers, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan. [06] Rokhila Juraeva, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.

Screening among collection microorganisms was conducted to identify producers of nanoparticles. Nanoparticles of silver were received at presence of active strains and were stabilized by chitosan of Bombyx mori. Structure and morphology of nanoparticles were studied by UV-spectroscopy and electron microscopy methods. It was established that synthesized nanoparticles of silver have spherical form and size ranging from 1.5 to 5 nm. Received data may be useful for further development of microbiological methods of nanoparticles production.

Microorganisms, Nanoparticles of Silver, Chitosan of Bombyx mori

[01] Ahmad, A., Mukherjee, P., Senapati, S., Mandal, D., Khan, M. I., Kumar, R., & Sastry, M. (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and surfaces B: Biointerfaces, 28(4), 313-318. [02] Baker, S., Harini, B. P., Rakshith, D., & Satish, S. (2013). Marine microbes: invisible nanofactories. Journal of Pharmacy Research, 6(3), 383-388. [03] Bochek, A. M., Vokhidova, N. R., Saprykina, N. N., Ashurov, N. S., Yugai, S. M., & Rashidova, S. S. (2015). The properties of chitosan-cobalt nanoparticle solutions and related composite films. Polymer Science Series A, 57(4), 460-466. [04] Ganbarov, Kh. G., Jafarov, M. M., Ramazanov, M. A., Agamaliyev, Z. A., & Eyvazova, G. M. (2017). Biosynthesis of silver nanoparticles using Saccharomyces sp. strain BDU–XR1. Environment, 4(6), 11-13. [05] Kalimuthu, K., Babu, R. S., Venkataraman, D., Bilal, M., & Gurunathan, S. (2008). Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids and Surfaces B: Biointerfaces, 65(1), 150-153. [06] Khandel, P., & Shahi, S. K. (2016). Microbes mediated synthesis of metal nanoparticles: current status and future prospects. International Journal of Nanomaterials and Biostructures, 6(1): 1-24. [07] Klaus, T., Joerger, R., Olsson, E., & Granqvist, C. G. (1999). Silver-based crystalline nanoparticles, microbially fabricated. Proceedings of the National Academy of Sciences, 96(24), 13611-13614. [08] Klaus-Joerger, T., Joerger, R., Olsson, E., & Granqvist, C. G. (2001). Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. TRENDS in Biotechnology, 19(1), 15-20. [09] Krutyakov, Yu. A., Kudrinskiy, A. A., Olenin, A. Yu., & Lisichkin, G. V. (2008). Synthesis and properties of silver nanoparticles: advances and prospects. Russ Chem Rev, 77(3), 233–257. [10] Mandal, D., Bolander, M. E., Mukhopadhyay, D., Sarkar, G., & Mukherjee, P. (2006). The use of microorganisms for the formation of metal nanoparticles and their application. Applied Microbiology and Biotechnology, 69(5), 485-492. [11] Narayanan, K. B., & Sakthivel, N. (2010). Biological synthesis of metal nanoparticles by microbes. Advances in colloid and interface science, 156(1), 1-13. [12] Pavani, K. V., Kumar, N. S., & Sangameswaran, B. B. (2012). Synthesis of lead nanoparticles by Aspergillus species. Pol J Microbiol, 61(1), 61-63. [13] Rashidova, S. S., & Milusheva, R. Y. (2009). Chitin and Chitosan Bombyx Mori. FAN, Tashkent. [14] Revina, A. A., Baranova, E. K., Mulyukin, A. L., & Sorokin, V. V. (2005). Some peculiarities of impact of cluster silver on yeast cells Candida utilis. Electronic scientific journal Studied in Russia, 1403-1409. [15] Sadowski, Z., Maliszewska, I. H., Grochowalska, B., Polowczyk, I., & Kozlecki, T. (2008). Synthesis of silver nanoparticles using microorganisms. Materials Science-Poland, 26(2), 419-424. [16] Vokhidova, N. R., & Rashidova, S. S. (2016). Synthesis and Stabilization of Cobalt and Cooper Nanoparticles by Chitosan Bombyx mori. Journal of Inorganic and Organometallic Polymers and Materials, 26(6), 1380-1386.