[01] George R. Ivanov, Laboratory of Nanoscience and Nanotechnology, Department of Physics, University of Architecture, Civil Engineering and Geodesy and Advanced Technologies Ltd., Sofia, Bulgaria.

The novel phenomenon of fluorescence self-quenching was earlier discovered by us. It was observed in monolayers from fluorescently head labeled phospholipids, mainly Nitrobenzoxadiazole Dipalmitoyl Phosphatidyl Ethanolamine (DP-NBD-PE), at the air-liquid interface. Here we report that the morphology and film structure is preserved during Langmuir-Blodgett (LB) film transfer on the solid support as observed with fluorescence microscopy. The self-quenching phenomenon in the solid phase is interpreted as radiationless energy transfer when NBD chromophores are below the critical distance R_c of 0,94 nm in the solid phase. Possible applications for high sensitivity and selectivity biosensors are discussed.

Fluorescence Self-Quenching, Langmuir Films, Langmuir-Blodgett Films, Phospholipids, Biosensors, Fluorescence Microscopy

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