[01] Helmy T. El-Zanfaly, Water Pollution Control Department, National Research Centre, Dokki, Cairo, Egypt.

Wastewater management has been subjected to different treatment technologies using chemical and biological techniques for several decades in order to protect the environment and public health. Biological treatment is based on microbial capability to reduce the pathogens load to achieve the lowest environmental as well as public health risks. However, such treatment is often neglecting the fact that bacteria resistant ability to antibiotics may increase during sewage treatment processes. This study aims to demonstrate the evidence of antibiotic resistant or multiple-antibiotic resistant (MAR) bacteria in water even after treatment, which can contribute to the increase their existence among the bacterial population. The study objective is extend to prove that the public health risk from the high existence of MAR population in drinking water begin to be much more complicated if the antibiotic resistance character is transferable from non-pathogenic bacteria to pathogens or opportunistic pathogens. The study showed that most bacterial species isolated from chlorinated water at two districts in Cairo, Egypt were resistance to ampicillin, sulfaguanidine and streptomycin. MAR bacteria represented 62.4 to 98% of the isolates. Antibiotic resistant bacteria could represent 40% to 70% of the isolates from the distributed drinking water. Bacterial isolates from wells at three water works in Cairo showed resistance to penicillin, 2-sulfanilamide pyrimidine, tetracyclin, chloramphenicol and neomycin. Study on the impact of activated carbon application in a pilot water treatment plant showed that the incidence of coliforms resistant strains among isolates varied significantly according to the source of water samples. MAR was not always high in the same samples in which the overall resistance was high. The antibiotic resistance character was mostly transferable. Accordingly and as a conclusion, the incidence of antibiotic resistant bacteria should be considered as valuable parameter in both wastewater treatment evalauation and in the new drinking water standards.

Wastewater Treatment, Antibiotic Resistant Bacteria in Water, Water Treatment Efficiency, Resistance Transfer

[01] American Public Health Association (APHA), 2005. Standard Methods for the Examination of Water and Wastewater. 21st Edn., Washington, D.C. [02] Armstrong, J.L., D.S. Shigeno, J.J. Calomiris and R.J. Seidler, 1981. Antibiotic-resistant bacteria in drinking water. Appl. Environ. Microbiol., 42: 277-283. [03] Ash, R.J., B. Mauck and M. Morgan, 2002. Antibiotic resistance of gram-negative bacteria in rivers, United States. Emerging Infectious Diseases, 8: 713-718. [04] Buchanan, R.E. and NE. Gibbons, 1974. Bergey’s Mannual of Determination Bacteriology, 8th Ed., The Williams and Wilkins Co., Bultimore, pp: 332. [05] Camper,A.K., M.W. LeChevallier, S.C. Broadway and G.A. McFeter, 1986. Bacteria associated with granular activated carbon particles in drinking water. Appl. Environ. Microbiol. 52: 434-438. [06] Dunnigan, A.P., 1969. Microbiological Control of Cosmetic Products. In: Federal Regulations and Practical Control Microbiology for Disinfectants, Drugs and Cosmetics. Soc. For Industrial Microbiol. Spec. Publ. [07] Elsas, J.D.V., J. Fry, P. Hirsch and S. Molin, 2000. Ecology of plasmid transfer and spread. In: Thomas, C.M., Editor. The Horizontal gene pool. Amsterdam, The Netherlands, Harwood, pp: 175-206. [08] Feary, S.T.W., A.B. Sturtevant and J. Lankford, 1972. Antibiotic resistant coliform in fresh and salt water. Arch Environ. Health, 25: 215-220. [09] Francy, D.S., E.E. Bertke, C.M. Kephart, R.A. Sheets and J. Rhoades et al., 2006. Spatial sampling and microbial source tracking tools for understanding fecal contamination at two Lake Eric beaches. U.S. Geological Survey Scientific Investigations Report, pp: 29. [10] Gaskins, H.R., C.T, Collier, D.B., Anderson. 2002. Antibiotics as growth promots: mode of action. Anim. Biotechnol., 13: 29-42. [11] Geldreich, E.E., 1991. Opportunistic organisms and the water supply convention. P. 823-842. In: Proceedings of the American Water Works Association. Water Quality Technology Conference. American Water Works Association. Denver. [12] Hanwood, V., M. Brownell, W. Penusek and J. Whitlock. 2001. Vancomycin resistant enterococcus spp isolated from wastewater and chicken feces in the United States. Appl. Environ. Microbiol., 10: 4930-4933. [13] Hirsch, R., T. Ternes, K. Haberer and K.L. Kratz. 1999. Occurrence of antibiotics in the aquatic environment. Sci. Total Environ., 225: 109-118. [14] Inversen, A., I. Kuhn, A. Franklin and R. Mollby, 2002. High prevalence of vanvomycin-resistant enterococci in Swedish sewage. Appl. Environ. Microbiol. 68:2838-2842. [15] Kummerer, K. (ED). 2008. Phamaceuticals in the environment. Sources, fate, effects and risk, third ed. Springer, Berlin, Heidelberg. [16] Kummerer, K., A. Henninger. 2003. Promoting resistance by the emission of antibiotics from hospitals and households into effluents. Clin. Microbiol. Infec. 9: 1203-1214. [17] Lamka, K.G., M.W. LeChevallier and RJ. Seidler, 1980. Bacterial contamination of drinking water supplies in a modern rural neighborhood. Appl. Environ., Microbiol. 39: 732-738. [18] Larsson, D.G., C., de Pedro, N. Paxeus. 2007. Effluent from drug manufactures contains extremely high levels of pharmaceuticals. J. Hazard. Mater, 148: 751-755. [19] LeChevallier, M.W., R.J. Seidler and T.M. Evans, 1980. Enumeration and characterization of standard plate count bacteria in chlorinated and raw water supplies. Appl. Environ. Microbiol., 40: 922-930. [20] Lennette, E.H., E.H. Spaulding and J.P. Truant (Eds). 1974. Manual of Clinical Microbiology, 2nd Ed., American Society for Microbiology, Washington, D.C., pp: 418. [21] Li, D., M. Yang, J. Hu, L. Ren, Y. Zhang, H. Chang, K. Li. 2008a. determination and fate of oxytetracycline and related compounds in oxytetracycline production wastewater and the recycling river. Ebvironm Toxicol, Chem. 27: 80-86, [22] Li, O., M. Yang, J. Hu, Y. Zhang, H. Chang, F. Jin. 2008b. Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river. Water Res. 42: 307-317. [23] Matsen, J.M. and A.L. Barry, 1974. Susceptibility Testing Diffusion Test Procedures. In: EH Mitsuhashi, 1977. Epidemology of R Factors. In S. Mitsuhashi, (Ed.). R factor drug resistance plasmid, University Park Press, Baltimore, Md., pp: 25. [24] Molstad, S., C.S. Lundburg, A.K. Karlsson, O. Cars. 2002. Antibiotic prescription rates vary markedly between 13 European countries. Scand. J. Infect. Dis. 34: 366-371. [25] National Research Council: Drinking Water and Health, V.I. 1977. Microbilogy of drinking water safe drinking water committee, advisory center on toxicology, assembly of life sciences. National Academy Sci. Washington, D.C., pp: 63-134. [26] National Research Council: Drinking Water and Health, V.II. 1980. An evaluation of activated carbon for drinking water treatment, safe drinking water committee, board of toxicology and environmental health hazards, Assembly of Life Sciences. National Res. Council. National Academy Press, Washington, D.C., pp: 251-380. [27] Niemi, M., M. Sibakov and S. Niemala, 1983. Antibiotic resistence among different species of fecal coliforms isolated from water samples. Appl Environm. Microbiol., 45: 79-83. [28] Pontes, D.S.D., F.A Pinheiro, C.I. Lima-Bittencourt, R.L Guedes and L. Cursino et al., 2009. Multiple antimicrobial resistance of gram-negative bacteria from natural oligotrophic lakes under distinct anthropogenic influence in a tropical region. Microb. Ecol., 58: 762-72. [29] Reasoner, D.J., 1991. Pathogens in drinking water-are there any new ones? P. 309-530 In: Proceedings of the American Water Works Association. Water Quality Technology Conference. American Water Works Association. Denver. [30] Reasoner, D.J. and E.E. Geldreich, 1985. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol., 49: 1-7. [31] Schwartz, T., S. Hoffmann and U. Obst, 1998. Formation and bacterial composition of young natural biofilms obtained from public bank-filtered drinking water systems. Water Res. 32: 2787-2797. [32] Schwartz, T., W. Kohnen, B. Jansen and U. Obst, 2006. Detection of antibiotic resistant bacteria and their resistance genes in wastewater, surface water and drinking water biofilms. FEMS Microbiology Ecology, 43: 325-335. [33] Stewart, M.H., R.L. wolfe and E.G. Means, 1990. Assessment of the bacteriological activity associated with granular activated carbon treatment of drinking watrer. Appl. Environ. Microbiol. 56: 3822-3829. [34] Thomas, K.V. 2008. The relevance of different point sources. Licture given at the "First international conference on sustainable pharmacy". 24-25 April. Asnabruck, Germany [35] USEPA, 2009. Water: Drinking Water Contaminants. National Primary Drinking Water Regulations. EPA, [36] USEPA, 2010. Proposed Revised Total Coliform Rule Assessment and Corrective Actions: Guidance Mannual, Draft, EPA, 815-D-10-001. [37] Walsh, C. 2000. Molecular mechanisms that confer antibacterial drug resistance. Nature, 409: 775-810. [38] WHO, 2008. WHO Guidelines fot Drinking Water Quality. 3rd Edn, WHO Geneva. [39] Witte, W. 1998. Medical consequences of antibiotic use in agriculture. Sci., 279: 996-997. [40] Wise, R. 2002. Antimicrobial resistance: priorities for action. J. Antimicrobiol. Chemoth. 49: 585-586. [41] Wolf, R.L., N.R. Ward and B.H. Olson, 1985. Inactivation of heterotrophic bacterial populations in finished drinking water by chlorine and chloramines. Water Res., 19: 1393-1403.