[01] Samaneh Zokae, Samaneh Zokae, MSC of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Iran. [02] Tahmineh Esfandiyari Sabzevar, Tahmineh Esfandiyari Sabzevar, PhD Scholare of Medicenal Plant, College of Agriculture, Islamic Azad University of Shirvan, Iran. [03] Raheleh Ahmadzadeh Ghavidel, Raheleh Ahmadzadeh Ghavidel, Phd of Agroecology, College of Agriculture, Ferdowsi University of Mashhad, Iran.

Curculigo orchioides is a perennial herb of the family Hypoxidaceae. It is an endangered plant species of medicinal importance. In this study a simple and efficient protocol for in vitro micropropagation of Curculigo orchioides. Through shoot tip and nodal portion explants and callogenesis of Curculigo orchioides through leaf, node and explants was used. A completely randomized design was followed for the experiment multiple shoots were obtained on all concentrations of BAP and NAA, but BAP (1 mg/l) showed the best response (90 and 80%) from both explants. Similarly, the best callus response was observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all explants. Then plantlets were transferred to different potting media in green house for acclimatization at the end of a three-month period, an average of 125 plants were obtained from a single meristem.

Curculigo orchioides, Micropropagaton, Plant hormones, Tissue Culture

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