[01] Kharoubi O., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria. [02] Benyamina A., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria. [03] Hallal N., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria. [04] Benyattou I., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria. [05] Aoues A., Faculty of Natural and Life Science, Department of Biology, Laboratory of BTE-BD-PR, University of Oran 1 Ahmed Benbella, Oran, Algeria. [06] Slimani M., Departments of Biology, University of Saida, Saida, Algeria.

Background: Aluminium (Al) poisoning is a potential factor in brain damage at even low and high levels, neurochemical dysfunction and severe behavioral troubles. Considering this effect, our study was carried out to investigate the effects of wormwood extract to restore enzymes activities, lipid peroxidation, carbonyl and behavioral changes induced by Al. Methods: twenty four Wistar rats were divided into four groups: one group was exposed to a mixture containing100 mg/kg body weight of AlCl₃ in the drinking with wormwood extract (Al+Pl), another group received wormwood extract (200 mg/kg body weight), other group received water after stopped intoxication for 5 weeks (Al(-)) and a groups as control. Activities of lactate deshydrogenase (LDH), Catalase (Cat), thiobarbituric acid-reactive substances (TBARS) and carbonyl level were determined in the whole brain of rats and the grooming and locomotors activity were defined in all groups. Results: The behavioral test (locomotors, sniffing and grooming test) indicates a significant hyperactivity in the Al+Pl group compared with the control group. After treatment with wormwood extract, the Pl indicates a lower activity compared with control and Al+Pl group.The intoxicated group (Al+Pl) has a significantly increased TBARS and carbonyl value compared to control (P < 0.05) and, after treatment with the wormwood extract, a significant reduction was noted. The LDH activity decreased significantly (P < 0.05) in the Al+Pl group compared with the control, by –39% and was increased in catalase activity by +196%. After wormwood extract administration, LDH and Cat activity were significantly changed compared to Al+Pl group. Conclusion: These data suggest that aqueous wormwood extract may play a very useful role in reduction of the neurotoxicological damage induced by Aluminium. Administration of wormwood extract may induce a neurochemical change and attenuate the alteration in neurobehavioral activity, observed in the development of age related disorders, such as Alzheimer’s disease.

Aluminium, Behavioral Test, Brain, Catalase, Carbonyl, Lactate Deshydrogenase, Lipid Peroxidation

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