Articles Information
International Journal of Preventive Medicine Research, Vol.1, No.5, Dec. 2015, Pub. Date: Jan. 6, 2016
Serological Survey of Avian Influenza Type a and Subtype a Antibodies in Chickens Sera in Sudan Using AGID and HAI Tests
Pages: 282-284 Views: 2620 Downloads: 1206
Authors
[01]
Wegdan H. A., Veterinary Research Institute (VRI), Khartoum, Sudan.
[02]
Khair S. A. M., Veterinary Research Institute (VRI), Khartoum, Sudan.
[03]
Mansour M. E., Veterinary Research Institute (VRI), Khartoum, Sudan.
Abstract
The aim of this study is designed to determine the prevalence of AI virus type A and subtypes H9, H7 and H5 antibody in different localities in Sudan. During the period of 2003 to early 2006, a total of 1025 sera samples were randomly collected from young, growing and adult, apparently health, unvaccinated chicken flocks at Khartoum, Central, Northern, Eastern and Western States of Sudan. Using AGID test, AI type A antibody was detected in 692 (67.51%) of the tested samples. From type A positive sera, a total of 311samples (36 sera from each state) were examined to subtypes H9, H7 and H5 by means of haemagglutination inhibition (HAI) test. AI antibodies to subtypes H9 and H7 were detected in 141(45.33%) and 11(3.54%) of tested serum samples respectively. All samples tested negative (0%) for type H5.The risk factors of location, Age groups and strains are statistically associated with seropositivirty of Avian Influenza ( P-value <0.05). In conclusion: The results obtained confirmed the presence of AI type A antibody and sub types H9 and H7 in Sudan.
Keywords
Avian Influenza, Sudan, Antibodies, AGID, HAI
References
[01]
Beard, C.W. (1970). Demonstration of type-specific influenza antibody in mammalian and avian sera by immunodiffusion. Bull. WldHlth Org. 42, 779-785.
[02]
Capua, I and Alexander, D. J. (2009). Ecology, epidemiology and human health implications of avian influenza virus infections. In. Avian influenza and Newcastle disease. A field and laboratory manual 1st edn.. Edited by Capua,I and Alexander, D.J. Springer- Verlag Italia . Chapter 1 Pp 1-18.
[03]
Gaffar Elamin, M. A. and Kheir, S.A.M. (1985). Detection of influenza antibodies in animal sera from Kassala region / Sudan by agar gel diffusion test. Rev. Elev. Medicine veterinary pays tropical, 38(2): 127-129.
[04]
Gough, R. E. (2004). Diagnosis of avian influenza, Proceedings the 11th International Conference of Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress 23-27 August, Sun way Pyramid Convention Centre, Petaling, Jaya, Malaysia. Universiti Putra Malaysia Press 144-146.
[05]
Manal, M. Elamin (2000). Studies on avian influenza in Khartoum, M.Sc. Thesis., Department of Preventive Medicine- Faculty of Veterinary Science, University of Khartoum-Sudan.
[06]
OIE (2015). Avian influenza. In: Manual of Diagnostic Tests and Vaccine for Terrestrial Animals, chapter 2-3-4 version updated May 2015.
[07]
Senne, D.A; Suarez, D.L; Stallnecht, D.E; Pedersen, J.C; Panigrahy, B. (2006). Ecology and epidemiology of avian influenza in North and South America. Dev Biol 124:37-44
[08]
Stegeman, J. A. and Bouma, A. (2004). Epidemiolgy and Control of Avian Influenza. The 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress 23-27 August, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. Universiti Putra Malaysia Press, 141-143.
[09]
Swayne, D.E. and Haivorson, D.A. (2003). Influenza. In Y.M. Saif, H.J. Barnes, A.M. Fadly, J.R. Glisson, L.R. Dougald and D.E. Swayne (eds). Disease of poultry 11th ed. Iowa State Press Ames. 135-160.
[10]
Swayne, D.E., Senne, D.A. and Beard, C.W. (1998). Avian Influenza: in D.E. Swayne, J. R. Glisson, M.W. Jackwood, J.E. Pearson and W.M. Reed (eds). Isolation and identification of avian pathogens, 4th ed. American Association of Avian Pathologist: Kennett square, PA, 150-155.
[11]
Terregino, C. and Capua, I (2009). Conventional diagnosis of avian influenza. In: Avian influenza and Newcastle disease. A field and laboratory manual 1st edn.. Edited by Capua, I and Alexander, D.J. Springer- Verlag Italia. Chapter 7Pp 73-85.
[12]
Wegdan, H. Ali., Kheir, S.A.M. (2007). Isolation of highly pathogenic avian influenza virus (H5N1) from poultry in Sudan in 2006. Journal of animal and veterinary advances 6(1):61-63.
[13]
Wegdan H. Ali; Khair, S.A.M. and Ballal. A. (2007). Serological Survey of Type A Avian Influenza Antibody in Chicken Sera in Sudan Using Indirect ELISA. Research Journal of animal and veterinary sciences, 2: 12-14.