[01] Olusegun Augustine Olusola, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria. [02] Isaiah Olabode Ogunsina, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria. [03] Oluremi Samson Ayedogbon, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria. [04] Olufunmi Adesayo Olusola, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria.

Lannea acida plants belong to the family Anacardiaceae. It has been used locally to treat and manage diseases that are contagious. Due to the recent increase in the resistance of malaria parasite to synthetic drugs the search for alternative treatment approach from plant sources is in urgent dimension. The antiplasmodial activity of mice was investigated with L. acida bark extracts. The study of the acute toxicity of the extract was used to determine lethal dose (LD₅₀). Suppressive activity of the extract was examined for five consecutive days with the extracts doses and chloroquine as the control drug, curative was infected for five days prior treatment, while the prophylactic groups were pretreated daily for five days before inoculation of 1×10⁷ chloroquine-sensitive Plasmodium berghei infected erythrocyte intraperitoneally. Five mice were in each group of five groups. Whilst, the Control group was administered with 10 ml distilled water/kg; 100, 200, and 400 mg extract/kg weight were administered for the experimental groups, and chloroquine 5 mg/kg body weight respectively. All doses of the extract produced substantial, dose reliant, chemo suppressive activity against the parasite in the suppressive, curative and prophylactic tests as compared with chloroquine treated mice. A lengthen mean survival time of the treated mice was observed as compared to the untreated mice. The oral median lethal dose (LD₅₀) of the extract in mice was 5000 mg/kg body weight. Empirical results of the study showed that the L. acida bark extract is harmless. It has a potent antimalarial property which could be of future importance in malaria management and antimalarial drug design.

Lannea acida, Antiplasmodial, Plasmodium berghei, Medicinal Plant

[01] World Health Organization (WHO) (2008). World Malaria Report, pp. 7-10. https://www.who.int/malaria/publications/atoz/9789241563697/en/ World Health Organization. [02] (WHO) (2016). World Malaria Report, pp. 13-15. https://www.who.int/malaria/publications/world-malaria-report2016/report/en/. [03] Roll Back Malaria (RBM, 2001). Roll Back Malaria Partnership: Malaria in Pregnancy. World Health Organization, 20 Avenue Appia, CH-1211 Geneva 7, Switzerland. www.rollbackmalaria.organisation...06/12/2010. [04] Sachs J, Malaney P (2002). The economic and social burden of malaria. Nature 415: 680-685. [05] Bassat Q, Mulenga M, Tinto H, Piola P, Borrmann S, Menéndez C, Nambozi M, Valéa I, Nabasumba C, Sasi P, Bacchieri A (2009). Dihydroartemisinin- piperaquine and artemether-lumefantrine for treating uncomplicated malaria in African children: A randomised, non-inferiority trial. PLoS One 4 (11): e7871. [06] Alonso PL, Brown G, Arevalo-Herrera M, Binka F, Chitnis C, Collins F, Doumbo OK, Greenwood B, Hall BF, Levine MM, Mendis K (2011). A research agenda to underpin malaria eradication. PLoS Medicine 8 (1): e1000406. [07] Bloland PB, Ettling M, Meek S (2000). Combination therapy for malaria in Africa: Hype or hope? Bulletin of the World Health Organization 78: 1378-1388. [08] Sutherland CJ, Ord R, Dunyo S, Jawara M, Drakeley CJ, Alexander N, Coleman R, Pinder M, Walraven G, Targett GA (2005). Reduction of malaria transmission to Anopheles mosquitoes with a six-dose regimen of co-artemether. PLoS medicine 2 (4): e92. [09] Bloland PB, Kachur SP, Williams HA (2003). Trends in antimalarial drug deployment in sub-Saharan Africa. Journal of Experimental Biology 206: 3761-3769. [10] Maroyi A (2018). Lannea acida: A review of its medicinal uses and Phytochemistry and pharmacological properties. Asian Journal of Pharmaceutical and Clinical Research 11: 11. [11] Banso A, Adeyemo S (2006). Phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri 18: 39-44. [12] Sofowora A (1993). Medicinal plants and traditional Medicine in Africa. (2^nd Edition), Spectrum Books limited. Sunshine House Ibadan, Nigeria. pp. 134-156. [13] Harborne JB (1993). Phytochemistry Academic Press, London. pp. 89131. [14] Trease G. E., and Evans W. C. (1989). Pharmacognosy, 11th edition. Bailliere Tindall, London pp. 45-50. [15] Boma O. T, Worlu-Wodo Q. E, Deekae S (2016). Bioprospective Screening of Ricinodendron Heudelotii seeds. Journal of Analytical and Pharmaceutical Research 3 (7): 00084. [16] Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, Atangbayila TO (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Tropical Journal of Pharmaceutical Research 7 (3): 1019-1024. [17] Okhale SE, Ugbabe EG, Bamidele O, Ajoku AG, Egharevba HO (2014). Phytochemical and antimicrobial studies on extractives of Calyptrochilum emarginatum (SW) Schltr (Orchidaceae) growing in Nigeria. Journal of Medicinal Plants Research 8 (4): 223-228. [18] Lorke D (1983). A new approach to practical acute toxicity testing. Archives of Toxicology 54: 275-287. [19] Peters W (1965). Drug resistance in plasmodium berghei. Vinka and Lips. Experimental. Parasitology., 17: 80-89. [20] Chees brough M (2004). District Laboratory Practice in Tropical Countries. 2nd ed. Cambridge University Press pp. 239-258. [21] Ryley JF, Peters W (1970). The antimalarial activity of some quinolone esters. Annals of Tropical Medicine and Parasitology 64: 209-222. [22] Saidu K, Onah J, Orisadipe A, Olusola A, Wambebe C, Gamaniel K (2000). Antiplasmodial, analgesic and antiinflammatory activities of the aqueous stem bark of Erythrina senegalensis. Journal of Ethnopharmacology 71: 275-280. [23] Adzu B, Salawu OA (2009). Screening Diospyros mespiliformis extract for antimalarial potency. International Journal of Biological and Chemical Sciences 3 (2): 271-276. [24] Abatan MO, Makinde MJ (1986). Screening Azadirachta indica and Pisum sativum for possible ant malarial activities. Journal of Ethnopharmacology 17: 85-93. [25] Pedroni HC, Bettoni CC, Spalding SM, Costa TD (2006). Plasmodium berghei: Development of an irreversible experimental malaria model in Wistar rats. Experimental Parasitology 113: 193-196. Peters W (1965). Drug resistance in plasmodium berghei. Experimental Parasitology 17: 80-89. [26] Pierrot C, Adam E, Laffite S, Godin C, Dive D, Capron M, Khalife J (2003). Age related susceptibility and resistance to Plasmodium berghei in mice and rats. Experimental Parasitology 104: 81-85. [27] Agbedahunsi JM (2000). Screening of crude drugs for the treatment of malaria in Nigeria, Phytomedicine in malaria and sexually transmitted diseases: Challenges for the new millennium. Drug Research and Production Unit, Faculty of pharmacy, Obafemi Awolowo University, Ile Ife, Nigeria. pp. 13-22. [28] Dahanukar SA, Kulkarni FA, Rege NN (2000). Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 32: 1081-118. [29] Thomas AM, Van Der Wel AM, Thomas AW, Janse CJ, Waters AP (1998). Transfection system for animal models of malaria. Parasitology Today 14: 248-249. [30] Etkin NL (1997). Antimalarial plants used by Hausa in Northern Nigeria. Tropical Doctor 27: 12-16.