[01] Okafor Ugochukwu Chukwuma, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria. [02] Nworji Kosisochukwu Emmanuela, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria. [03] Obubu Maxwell, Department of Statistics, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.

The research was aimed at determining the sensitivity and/or resistance of isolated microbes from swimming pools to the various antimicrobial drugs in the case of infections arising from use of infected pools. Water samples were aseptically collected from ten different outdoor swimming pools and microbiologically analysed using standard methods. Using standard disc diffusion method, the antimicrobial susceptibility pattern of isolates was determined against antibacterial agents (Rocephin (30μg), Pefloxacin (10μg), Gentamycin (10μg), Ciprofloxacin (10μg), Streptomycin (30μg), Ampiclox (30μg), Septrin (30μg), Zinnacef (20μg), Erythromycin (19μg), Amoxicillin (30μg)) and antifungal agents (Nystatin, Fluconazole, Ketoconazole). The isolates include; Bacillus spp, Proteus spp, Pseudomonas spp, Salmonella spp, Staphylococcus spp, Citrobacter spp, Klebsiella spp, Escherichia coli, Enterobacter spp, Aspergillus niger and Fusarium spp. Statistical analysis showed there was no significant difference in the microbial count between the 10 different outdoor pools sampled. The effectiveness of the antimicrobials to the isolates were defined in percentage as follows: Rocephin (7.7%), Pefloxacin (10.9%), Gentamycin (9.3%), Ciprofloxacin (8.8%), Streptomycin (10.4%), Ampiclox (9.5%), Septrin (11.0%), Zinnacef (10.7%), Erythromycin (11.8%), Amoxacillin (10.0%) and Nystatin (37.0%), Fluconazole (36.0%), Ketoconazole (27.0%). With respect to the antimicrobial susceptibility patterns of the isolates, results showed that most of the test antimicrobials were effective against the microbes, with Erythromycin and Nystatin being the most effective for the bacteria and fungi isolates respectively. Thus, Erythromycin and Nystatin should be the first line of prescription in cases of swimming related infection and disease. It should be noted that bacterial and fungal resistance was seen among drugs such as Rocephin and Ketoconazole respectively.

Antibiotics Resistance, Microorganisms, Swimming Pool, Microbial Count, ANOVA

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