International Journal of Bioinformatics and Biomedical Engineering
Articles Information
International Journal of Bioinformatics and Biomedical Engineering, Vol.4, No.4, Dec. 2018, Pub. Date: Dec. 6, 2018
Enzymatic Potentials of Lactic Acid Bacteria Isolated from Palm Wine
Pages: 56-61 Views: 35 Downloads: 24
Authors
[01] Obi Clifford Nkemnaso, Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Umuahia, Nigeria.
Abstract
The enzymatic potentials of Lactic acid bacteria (LAB) isolated from fresh palm wine in Ikwuano L. G. A namely Umuariaga, Amawom, Oboro and some markets around Umuahia North and South in Abia State were evaluated. Fresh palm wine samples were collected from the tappers between 6 and 9am using sterile labeled bottles. The bottled were packed in ice packed container and quickly transported to the laboratory for analyses. The samples were serially diluted and inoculated by spread plate method in duplicates onto De Man Rogosa Sharpe (MRS) Agar and incubated at 35°C for 48 hrs for the isolation of LAB. Six LAB isolates namely Lactobacillus plantarum, Lactobacillus pentosus, Lactobacillus brevis, Lactobacillus fermentum, Lactococcus spp and Leuconostoc spp were recovered from fresh palm. Result showed that all the isolates gave positive amylase, protease and lipase activities. The optimum temperature for enzymatic activity was 50°C for the three enzymes while the optimum pH was in the range 5.0. At the optimum temperature, amylase activity was 9% at pH 5.0, lipase activity was 5.33% and protease activity was 6.14%. The LAB isolates also demonstrated resistance to amoxyl and tetracycline antibiotics. It was concluded that palm wine is a good source of different species of Lactic Acid Bacteria and as such has the potential for increases utility value as raw materials for industries in sourcing for different enzymes that can work at thermophilic temperatures typical of industrial setting.
Keywords
Activity, Enzymatic Potential, Fermentation, LAB, Palm Wine
References
[01] Ukhun, M. E., Okolie, N. P., Oyerinde, A. O. (2005). Somemineral profiles of fresh and bottled palmwine – acomparativestudy. African Journal of Biotechnology. 4 (8): 829-832.
[02] Adebayo, M. R., Ajiboye, A. E. (2011). Antimicrobial property of palmwine. International Journal of Microbiology Resources. 2 (8): 265-269.
[03] Bohoua, G. L. (2008). Effect of palmwine yeast and yoghurtprobiotics on the growth performance of broilers. Livestock Resource and Rural Development. 20 (3).
[04] Obire O. (2005). Activity of Zymomonas species in palm sapobtained from three areas in Edo state, Nigeria. Journal of Applied Sciences and Environmental Management. 9: 25-30.
[05] Nester, E. W., Anderson, D. G., Roberts, C. E., Pearsall, N. N., Neater, M. T. (2004). Microorganisms in food andbeverage production – alcoholic fermentation by yeast. In: C. H. Wheatley (ed) Microbiology – A Human Perspective, 4th Edition. McGrawHill, NewYork. 151-153.
[06] Ogbulie T. E., Ogbulie J. N., Njoku H. O. (2007). Comparative study on the microbiology and shelflife stability of palmwine from Elaeis guineensis and Raphia hookeri obtained from Okigwe, Nigeria. African Journal of Biotechnology. 6 (7): 914-9
[07] Ezeagu, I. E and Fafunso, M. A. (2003). Biochemical constituents of palm wine. Ecology of Food and Nutrition. 42: 213-222.
[08] Nwokeke, N. V. (2001). Palmwine Preservation Using Traditional Plants that have preservative bases. B.Sc. Thesis. Imo State University, Owerri, Nigeria.
[09] Heller, K. J. (2001). Probiotic bacteria in fermented foods: product characteristics and starter organisms. American Journal of Clinics and Nutrition. 73: 374-379.
[10] Ezereonye, O. U. (2004). Nutrient utilization profile of Saccharomyces cerevisiae from palm wine fruit fermentation. Antonievan Leeuwenhoek. 86 (3): 235-240.
[11] Cheesbrough, M. (2014). District laboratory practice in tropical countries part 2. Cambridge University Press.
[12] Sanni, A., Morlon-Guyot, J., Guyot, J. P. (2002). New efficient amylase-producing strains of Lactobacillus plantarum and L. fermentum isolated from different Nigerian traditional fermented foods. International Journal of Food and Microbiology. 72: 53–62.
[13] Nwachukwu, I. N., Ibekwe, V. I., Nwabueze, R. N., Anyanwu, B. N. (2006). Characterisation of Palm wine yeast isolates for Industrial Utilization. African Journal of Biotechnology. 5 (19): 1725-1728.
[14] Tchekessi, C. C., Bokossai, I. Y., Azokpota, P., Agbangla, C., Daube, G., Scippo, M. L., Korsak, N., Gotcheva, V., Blagoeva, G., Angelov, A. (2014). Isolation and identification of Lactic Acid Bacteria from traditional fermented products in Benin. International Journal of Current Microbiology and Applied Sciences. 3: 1-8.
[15] Guyot, J. P. (2010). Fermented cereal products in Tamang, J. P editor. Fermented foods and beverages in the world. London: CRCPress (Taylor and Francis Group). 247-261.
[16] Mapp, C. E.. (2001). Agents, old and new, causing occupational asthma. Occupational Environmental Medicine. 58 (5): 354-360, 290.
[17] Metzger, J., Bornscheuer, U., (2006). Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification. Applied Microbiology and Biotechnology. 71: 13-22.
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