[01] Muhammad Ali Akond, Department of Botany, Jahangirnagar University, Savar, Dhaka. [02] Mst Nusrat Jahan, Department of Botany, Jahangirnagar University, Savar, Dhaka. [03] Nigar Sultana, Legend College, Sobhanbag, Jaleshor, Savar, Dhaka. [04] Farhana Rahman, Appartment No. 1405, Building No. 20, Japan Garden City, Mohammadpur, Dhaka.

The effect of physiological parameters viz., temperature, pH and NaCl on actinomycetes strains belonging to the genus Streptomyces (JUBM-35-NS-1) and Nocardia (JUBM-35-NS-2), isolated earlier from straw and compost materials used in commercial mushroom cultivation, was studied. Growth maxima were observed at 30°C and 35°C respectively in Streptomyces sp. and Nocardia sp. Arrest in growth for both isolates was found to be at 4°C and 50°C temperatures. The optimum growth was recorded at pH range of 6.0-6.5 for the isolate JUBM-35-NS-1 while it was at 6.5-8.0 for isolate JUBM-35-NS-2. Maximum growth was shown at NaCl concentration of 0% and 0.75% respectively by the isolate JUBM-35-NS-1 and JUBM-35-NS-2. The NaCl tolerance for growth of isolate JUBM-35-NS-1 was up to 8% and that for the isolate JUBM-35-NS-2 was up to 2.0%. A lower concentration of NaCl was found stimulatory with decline over 1.5% and complete cessation at 2.5% for the isolate JUBM-35-NS-2 (Nocardia sp.). The isolate JUBM-35-NS-1 (Streptomyces sp.) was found to be salt tolerant showing a stable growth from 0.5% to 8.0% with complete arrest of growth at 8.5% of NaCl concentration in culture medium.

Actinomycetes, Growth, Temperature, pH, Salinity

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