[01] Zhizhi Zhang, College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China; Jiangsu Co-innovation Center for Preventiom and Control of Important Animal Infectious and Zoonoses, Yangzhou, P.R. China. [02] Rui Wei, College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China; Jiangsu Co-innovation Center for Preventiom and Control of Important Animal Infectious and Zoonoses, Yangzhou, P.R. China. [03] Jingyi Wu, College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China; Jiangsu Co-innovation Center for Preventiom and Control of Important Animal Infectious and Zoonoses, Yangzhou, P.R. China. [04] Siyue Zhou, College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China; Jiangsu Co-innovation Center for Preventiom and Control of Important Animal Infectious and Zoonoses, Yangzhou, P.R. China. [05] Tangjie Zhang, College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China; Jiangsu Co-innovation Center for Preventiom and Control of Important Animal Infectious and Zoonoses, Yangzhou, P.R. China.

The OASL gene is one of the interferon-stimulating genes whose protein family includes OAS1, OAS2, OAS3 and OASL. 12 Muscovy duck from one pure line was employed for testing. 5'-regulatory region of the Muscovy duck OASL gene was amplified. After sequencing of PCR product, the SNPs were identified and the polymorphism of OASL gene sites could be detected by Popgene and SHEsis. The results showed 11 SNPs (g.313A>G, g.354A>G, g.392T>C, g.423A>G, g.450T>C, g.523G>A, g.528T>C, g.533C>T, g.545A>G, g.604A>T, g.609+>-), consisting 1.3% of total analyzed sites. The mutations were equally distributed which demonstrate low overall heterozygosity and strong linkage disequilibrium. All of the study specified the mutation types and locations on part of the OASL gene, paving the way for the development of gene regulation and the mechanism of antiviral actions of OASL gene and providing related basic dataand information of disease-resistance breeding of poultry.

Muscovy Duck, OASL Gene, Single Nucleotide Polymorphism, Bioinformatics Analysis

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